Learning and teaching are the concerns of trained teachers. The more senses are stimulated in the teaching process, the more learning will occur. Learning can be reinforced, made interesting and memorable for learners with the use of instructional materials. This study investigated, "The Use of Instructional Materials in Nurse Education: Actual use, Barriers and Attitudes: The Nurse Teachers' Perspective." The general objective of the study was to assess the use of five instructional materials in nurse education. The descriptive survey research design was used for this study. Out of a target population of 105 nurse teachers in North West and South West Regions, 91 were purposefully selected as the sample. A questionnaire was administered and 76 respondents responded giving a response rate of 84%. Data were analysed using the Statistical Package for Social Science, version 17 (2010, Atlanta Georgia). The results showed that the teachers who have been teaching longer (57.9%) actually used instructional materials. More teachers, (53.9%) were found in government nursing schools than in private schools (46.1%). The results also showed that the chalkboard, manikins, handouts, flipcharts and power point are actually being used, scoring 74%, 57%, 51%, 51% and 55% respectively, with barriers such as: expensive to own, expertise skills required, lack of time and difficult to produce, that impede their use and affect attitudes towards their use. The results mean that the five selected instructional materials are actually being used by nurse teachers and need encouragement from government and private proprietors in their purchase, in order to make them always available for use.