The purpose of this quasi experimental research 2 x 3 was to reveal the effect of JiRQA learning strategy on critical thinking skills of multiethnic students in higher education. This research conducted on zoology lecture, in the even semester of the 2015/2016 academic year. The samples of this research were 125 students consisting of ethnics of Dayak, Javanese and Malay. The data of the critical thinking skills were obtained from the pretest and posttest using essay test, and analyzed by using analysis of covariance (ANCOVA) at the level of significance 5%, followed by Least Significant Difference test (LSD). The results of the data analysis show that the strategy, ethnic and the interaction between the strategy and ethnic have a significant effect on the student critical thinking skills. The students' critical thinking skills in JiRQA strategy are 20.59% higher and significantly different than that of the conventional learning. The critical thinking skills of Javanese students are 11.56% higher than those of the Malay students. There is no significant difference of student critical thinking skills between Malay and Dayak students, as well as between Javanese and Dayak students. Student critical thinking skills in JiRQA learning strategy are not significantly different among ethnics, but those are significantly different among ethnics in conventional learning. All combination groups of conventional learning are significantly different among each other, where the combination group of Javanese is the highest one.