People with Spinal Cord Injury (SCI) are susceptible to several secondary problems, such as pressure ulcers, urinary infections, and a high risk of depression. Otherwise, it has been widely accepted that participation in sport may provide opportunities for people with disabilities, with the potential to promote quality of life demonstrated by psychological functioning. This study aimed to evaluate and compare the psychological well-being of Paralympic Athletes with SCI (PA), Non-Athletes with SCI (NA) and Healthy Volunteers (HV), assessed by SF-36. This is a comparative, descriptive, and qualitative study. The protocol for carrying out this research followed the Standards for Reporting Qualitative Research (SRQR). After inclusion and exclusion criteria, forty people were asked to complete the informed consent form and the questionnaire. Then, they were divided into three groups: PA, NA, and HV. The data of this study demonstrated that the Vitality, Social Functioning, Role Emotional Limitation, Mental Health, and Mental Composite Score were higher in PA group, as compared to NA, and lower in Physical Functioning compared to HV group. Finally, strengthening the engagement in Paralympic sport among people with SCI is a promising strategy to promote psychological well-being and decrease susceptibility to several secondary problems in this vulnerable group.