Vitamin E is widely used as a drug supplement in many diseases.The aim of these investigations was conducted to study vitamin E supplement effect on testicular tissues in male albino rats after 90 days of repeated treatment and after stopping treatment for 45 days. Sevengroups of six male rats were orally administered 500, 1000 and 2000 mg/kg body weight vitamin E daily and the control received distilled water for 90 days. Testosterone and histological examination of testes were assessed. The elevation of the number of seminiferous containing sperm and not containing sperm was counted. The rats treated with two higher Vitamin E supplement dose treatment caused a significant increase in testosterone and seminiferous containing sperm. Therefore, oral administration of vitamin E supplement in rats for 90 days was safe for the testis and has a spermatogenic effect. The androgenic effect of vitamin Epersists even stopping treatment.