As helpful as ATM machine is, recently id has been identify to have a detrimental effect on the health of its users, as it is now a source of infection to the users. Bacteriological examinations were carried out on cash dispensing machine (ATM). Samples (dusts) were taken from the ATM key-pads of some selected banks at Ado-Ekiti, Nigeria. The samples were collected with sterile cotton swab and then placed on culture media using streak-plating techniques. The isolates were identified using biochemical and molecular characterization techniques. Antibiotic susceptibility tests were done using agar diffusion method. Also plasmid analysis was carried out. Biochemically, percentage distribution of the isolates revealed high population of Pseudomonas aeruginosa (50%), Escherichia coli to be (30%) and Proteus vulg two strains possessed two plasmids each with molecular weight of 1.710kbp and 1.415kbp and were characterized molecularly to be strains of Eubacterium siraeum and Enterobacter tabaci. The health implication of the isolated strains on the environment and ATM users is discussed.