This study evaluated the microbial load of plantain chips sold in Yenagoa metropolis, Bayelsa state, Nigeria. Triplicate sample of plantain chips were purchased from market area in Akenfa, AgudamaEpie, Etegwe junction, Igbogene, Kpansia and Opolo. The microbial population was enumerated using pour plate method. Results of the total heterotrophic bacteria, bacteria of the Enterobacteriaceae family and total fungi ranged from 3.99 - 4.73 Log cfu/g, 2.46 - 2.84 Log cfu/g and 2.90 - 3.38 Log cfu/g, respectively. Analysis of variance revealed no significant variation (P>0.05) among the different locations. The populations were within tolerable limit for ready to eat food as specified by Food and Agricultural Organization, Food Quality Check Programme and International Commission on Microbiological Specifications for Foods. Based on the density of the bacteria of the Enterobacteriaceae family there is the need to improve handling processes.