The problems of microbial resistance to the available antibiotics especially in the hospitals are fast growing and causing a lot of concern. The study was aimed to identify and evaluate antibiotic susceptibility pattern of clinical isolate of methicillin resistant Staphylococcus aureus (MRSA) isolated from wound, HVS and urine sample of patients attending some tertiary hospital in Kano, Nigeria. A total of 107 (37 from wounds and pus, 29 from High vaginal swab (HVS), 41 from urine) suspected clinical isolates of Staphylococcus were collected from the study hospitals over a period of eight months (October, 2015 to May, 2016). Staphylococcus aureus were characterized using conventional microbiological methods: Gram staining, Biochemical test (Coagulase test, Catalase test and DNase test) and Mannitol fermentation test. Disc diffusion method using oxacillin and cefoxitin sensitivity discs were employed for the identification and evaluation of antibiotic susceptibility pattern of MRSA. The results showed that Staphylococcus aureus were able to ferment Mannitol and positive for Catalase, Coagulase and DNase test. MRSA isolates were resistant to beta lactam drugs including both penicillins and cephalosporins. It also showed resistivity to macrolide, and fluroquinolones, but sensitive to tetracycline and aminoglycoside.