Microorganisms like bacteria and fungi are capable of degrading a wide range of industrial effluents and environmental pollutants. Use of microorganisms to degrade toxic chemicals is a natural and environment friendly method. Dimethoate is an organophosphorous pesticide. In the following study, dimethoate degrading microorganisms have been isolated from various industrial effluents. The isolated organisms have been characterized by various biochemical tests. Standardisation of degradation was performed using Leuco crystal violet reagent as indicator of degradation and colorimetric analysis was performed at 595 nm. 6 different effluent samples were used. 8 different isolates were got. 4 isolates belonged genus Bacillus, 2 belonged to genus Staphylococcus and 2 organisms were fungi belonging to the genus Aspergillus.