The purpose of the routing protocols in networks is to find the shortest path for transferring the data and enhancing the overall throughput of the network. The paper emphasizes on conducting practical implementation of ACO algorithm to find out the shortest path under different scenarios differentiating in number of iterations, number of ants, and evaporation rate. The implemented algorithm may be used in large network with heavy loads in selecting the most appropriate path.Moreover, with the increase in the number of iterations, the chances of controlling the congestion effectively also increases.