Oleaginous microorganisms (Thraustochytrids) are typical marine fungi that produce docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which are ω-3 polyunsaturated fatty acids (PUFAs). The use of microbial DHA and EPAs is rapidly improving human health and therefore arousing several clinical trials. To cultivate Thraustochytrids, the technique used is very important. A number of researches suggested that DHA and EPA ameliorate many health problems including cardiovascular disorders, rheumatoid arthritis, different types of cancers and alzheimer's disease and so many , which are thus used for pharmacological and as well as nutraceutical purpose.