Urine contains different types of chemicals called as nitrates. The entrance of bacteria in the urinary tract cause the conversion of urine nitrates into nitrites. The bacteria which cause the infection of urinary tract are E. coli and some gram-negative bacteria. Cleft chin or dimple on the chin can be inherited from the parents or grandparents. This is the Y shaped cleft on the chin that is caused by the genetics in humans. Muscle of the mentalis fails to fuse and it leaves a gap. The main objective of this study is to correlate the urine nitrates with dimple on the chin. A total of 100 subjects participated in the research. All the subjects were undergraduate students in Bahauddin Zakariya University, Multan, Pakistan. The relation of nitrates in urine with chin dimple was observed. Urine test was done to check the nitrates level. It was inferred from the study that people with absence of chin dimple has high ratio of absence of urine nitrates in them. So there is no relation of urine nitrates with the dimple on the chin.