Yellow colored substance is formed when the RBCs breakdown. This yellow color substance is found in liver and excreted out of body during absorption. Chin dimple is a mendelian trait and it can be passed from parents to offspring. The main objective of this study was the relation of chin dimple with urine bilirubin. A total of 100 subjects participated in this activity and all the subjects were student in Bahauddin Zakariya University, Multan, Pakistan. Urine bilirubin was observed by the urine test. It was concluded from the study that highest %age of normal value of bilirubin was in people with absence of chin dimple in them so there was no relation of dimple with urine bilirubin.