A cross-sectional study was conducted from December 2011 to April 2012 to estimate the prevalence of Bovine trypanosomosis to identify the dominant trypanosomes species, to assess vector density magnitude and to identify the species of tsetse fly (Glossona species) in Kindo Koysha woreda, Wolaita zone, Southern Ethiopia. The study involved 229 local cattle of different age group and both sexes. The buffy coat method was used for determination of prevalence and identification the trypanasoma species and NGU and biconical traps baited with attractants (acetone cow urine) were deployed to study the vector density. The overall prevalence of trypanasomosis was 6.1%. Trypanasoma congolense and T.vivax were the species identified with 4.4%, 1.8% prevalence respectively. All potential risk factors included in the study: age, sex, coat color, body condition and Kebele (locality) were not significantly associated (P>0.05) with trypanasoma status of the study animal. Mean PCV of parastemic animals (20.9%) was lower compared to mean PCV of (24.1%) aparasitemic cattle (p< 0.01) the overall apparent density of tsetse and biting flies were 0.2 and 0.12 flies per trap per day respectively. The present study revealed that bovine trypanasomosis is still endemic and causes production loss in the study area despite attempts to control it. Measures which may help reduce the effect of trypanasomosis are suggested.