Bovine anaplasmosis is one of a vector borne disease of cattle which cause a major problem on the health and production of cattle in tropical and sub-tropical regions. A cross-sectional study was carried out from November 2016 to April 2017 on cattle population to determine the overall prevalence of bovine anapalsmosis and its associated risk factors in and around Wolaita Sodo town. A total of 384 blood samples were collected from randomly selected cattle in the study area. Age, sex, origin, breed, body condition, tick infestation and previous treatment were considered as risk factors in the study. Giemsa stained thin blood smears were prepared and examined under oil immersion lens (100x) of microscope for the presence of anaplasma based on morphology and position within RBC. An overall prevalence of bovine anapalsmosis was 11.46% and A.marginale was relatively most prevalent species (7%), then A.centrale (2.6%) and co-infection of both species (1.8%). The variation of animal origin with the prevalence of disease was not statistically significant (P>0.05). In addition the association of breeds, sex, age and body condition with the prevalence of anaplasmosis was not significant (P>0.05). Animal infested with tick was more exposed to the infection of the disease than that of non infested animals. As a result tick infestation was significantly (p < 0.05) associated with the occurrence of anaplasmosis. Similarly, the prevalence of anaplasmosis was significantly (p < 0.05) related with animals that have history of previous injection within two months before sample collection. Anemia (PCV< 24) was also significantly correlated with incidence of bovine anaplasmosis compared with non anemic animals (PCV = 24). In conclusion, bovine anaplasmosis is one of the important tick borne diseases of cattle that present in the study area. Therefore, appropriate disease control measures should be done to improve livestock production.