Mycorrhizal status of protected forest, agroforestry practices, and mono-cropping lands were investigated in Wensho district of Sidama Zone in Ethiopia. Rhizosphere soils and root samples were analyzed for soil physicochemical properties, spore density, & AM-root colonization following standard methods. Except some non-mycorrhizal groups, all plants surveyed in the three land-use types showed arbuscular mycorrhizal colonization, indicating a high mycorrhizal dependency of plants in this environment. A total of 26 AMF morphospecies, belonging to seven genera (Acaulospora, Entrophospora, Glomus, Claroideoglomus, Funneliformis, Scutellospora and Gigaspora), were found in the rhizospheres of selected plants in the three land uses. Spores of three genera Glomus, Funelliformis and Acauolospora had higher spore production, accounting for 58.10%, 17.70% and 9.29% of the total number of spores respectively. Oneway analysis of variance (ANOVA) showed that spore density and root colonization of different AM structures varied greatly among plant species both within and between different land-use types. Spore density was higher in culturally protected forest and AM colonization was higher in the agroforest. The lowest number of spores and the lowest percentage of root colonization were recorded in cropland. When land use types were considered separately or together no significant correlation between spore densities and AM colonization was observed. Cluster analysis based on the similarity in AM status with respect to spore density showed no similarity except for particular species in different land uses. Research result indicates that mono-cropping reduces spore density and AM colonization in comparison with the culturally protected forest and agroforest.