It is established fact that, the Pycnocycla bashagardiana a traditional endemic plant utilizing for its essential oil to several beneficial purposes are for flavoring materials, but very few work were done with regarding to its anti-bacterial and antioxidant activities. After extraction of essential oil from the plant, the GC and GC/MS analyses were used. The oil composition estimated in the identification of 22 compounds through both techniques. The Myristicin was recorded of 69.54%. As well as, the E-Ocimene, Z-Ocimene and Methyleugenol were the main components by estimating of 21.04%, 9.24%, and 4.70% respectively. The obtained results showed that the essential oil revealed the antimicrobial properties against gram-positive and gram-negative. It was also observed against gram-negative bacteria revealed high resistance and antifungal properties against fungus alternaria, for some extend over all, this essential oil revealed a high resistance to said respected organisms. Moreover, the antioxidant activity was performed by adopting the DPPH method, through which the Pycnocycla bashagardiana essential oil indicated highly significant radical scavenging antioxidant activity of IC50=3.08±0.368 mg/ml.