The resistance of bacteria to antibiotics has been a current topic of public health. Currently, it is known that a variety of bacteria are resistant to antibiotics. Therefore, alternative strategies are being sought that inhibit bacterial growth and that function as bactericides for the treatment of bacterial infections. Some extracts and essential oils from plants seem to be the solution because they have powerful bactericidal properties, they are easy to obtain, they do not pollute the environment, the production cost is low. It has been reported also the bacterial antibiofilm activity from plant products or extracts rich in bioactive compounds and that they act on a variety of pathogenic bacteria. This work shows the effect of essential oil of Thymus vulgaris on the formation of biofilm from uropathogenic E. coli in venoclysis tubes.