The uniqueness of the Lonar Lake water is its salinity and alkalinity. Most of the industrial processes are carried out at high temperature and at high pH and needs enzyme having high temperature and pH stability. The present study deals with isolation, production and dynamics of amylase from bacterial strain isolated from the alkaline Lonar Lake. Isolation of bacteria was done by using Horikoshi medium and screened for production and partial characterization of amylase. A total of five bacterial cultures were isolated and a bacterial isolate DHT 18 selected for study was characterized by cultural, morphological, biochemical and 16S rRNA gene sequencing resulted it into Lysinibacillus xylanilyticus which showed optimum activity at temperature 600C and pH 10 indicating thermo-stability which is most useful in food, pharmaceutical and detergent industries and can be exploited for biotechnological potential.