This article discusses about the various growth parameters of B.aerius and B.cereus in context of their probiotic capabilities. The optimum temperature for growth for the isolates was 37°C and 45°C respectively and pH 6 for both. The isolates sporulated efficiently in a nutrient depleted medium. B.cereus was non hemolytic in nature while B.aerius was rendered non hemolytic. Tolerance to gastric acidity and bile salts is considered as an important factor for the probiotic to exert beneficial health effects. Resistance to pH 2-3 was observed for both the isolates with the ability of B. aerius spores to germinate at pH 3. Also the spores could tolerate bile salt concentration up to 2%, with an ability to germinate at lower concentration. The present study thus proves the probiotic potential of both the Bacillus species.