One of the problems associated to bacterial biofilms is the extraordinary resistance to biocides and antibiotics. The biofilm formation gives advantages to bacteria as the natural protection from the environment and the host defense mechanisms. It has been reported that the bacteria are forming biofilm adhered to the walls of water pipes. The bacterial biofilm formation in the water pipes has been of interest in many countries because are pathogenic microorganisms for what constitutes a threat to public health. The present study aimed to seek the antibiotic resistance by bacteria to form biofilm into water pipes commonly used.