The experiment was carried out to assess the effects of agro morphological traits on quantity and quality of oil productions of Ethiopian mustard landraces at Holetta Agricultural research Center, Ethiopia. Therefore forty nine genotypes of Ethiopian mustard land races collected from different agro ecologies were evaluated to assess the effect of agro morphological traits on quantity and quality of oil traits produced. The experiment was carried out in a simple lattice design. Univariate analysis of variance showed that there were significant differences among genotypes for all agro morphological traits, quantity and oil quality related traits compared. The significant difference indicates the existence of genetic variability reflection in genetic effects of agro morphological traits among the accessions which is important for improvement of these traits. The present study revealed the presence of considerable variability among genotypes for all traits assessed. The significant difference of results indicates that the presence of good opportunity to improve these agro morphological, oil yield quantity and quality related traits of Ethiopian mustard using the tested genotypes.