Barley is one of the founders, old world agriculture and the first domesticated cereal crop. This experiment was conducted on fifteen barley genotypes against checks at Haro Sabu Agricultural Research Center sub site in 2017-2018.The objective was to assess the magnitude of genotype by environment interaction, grain yield stability, high yielding, insect pest tolerant genotypes in the growing areas of western Oromia. Seeds were planted in RCBD with three replications. Eleven agronomic traits and four disease reaction data were evaluated. Analysis of variance detected significant difference among genotypes for most observed traits both separated and combined analysis. Observation attained significant differences over years and locations for almost all traits. The combine ANOVA and the AMMI analysis for grain yield across environments revealed significantly affected by environments, that hold 41% of the total variation.The genotype and genotype by environmental interation, were significant and accounted for 10.46 % and 27.69 % respectively. Pricipal component 1 and 2 accounted for 15.74 % and 5.71 % of the GEI respectively with a total of 21.45 % variation.In general, G2 and G12 were identified as the best genotypes for yielding ability, stability, tolerant to diseases and use as parents in future breeding programs.