The experiment was conducted to determine harvesting cycle and appropriate number of tiller per planting slip using two different Lemmon grass varieties for better herbal and essential oil yield during 2017/2018 cropping season at Wondo Genet Agricultural Research Center. The design of the experiment was factorial RCBD design in three replications and the treatments of the experiment include combination of two Lemmon grass varieties with four level of number of tiller per planting slip (one, two, three and four tiller number per planting slip). Data on yield and yield components were collected and subjected to analysis of variance using SAS software version 9.3. Differences between means were assessed using least significant difference at 5% probability level. Results of the experiment showed that the interaction factors (Lemmon grass variety and number of tillers per planting slip) were non-significant for all parameters. However, there was significant variation between two Lemmon grass varieties in plant height, number of leaf per hill, fresh leaf weight, and essential oil yield where the higher values were recorded at WG-Lomisar-Java variety. Between the first and second harvesting cycles, there was variation in plant height and the higher values (133.79cm) were obtained at second harvest. Moreover, due to variation in number tiller per planting slip, there were significant and decreasing trend as number of tillers per planting slip increased from one to four for plant height, number of leaf per hill, number of tiller per plant, essential oil content, and leaf fresh weight while there was increasing trend for survival count. Therefore, we recommend variety WG-Lomisar-Java and planting slips having fewer number of tiller for fresh leaf weight and essential oil yield. For more a comprehensive result, the experiment should be repeated and more harvesting cycles should be considered further.