The current work was carried out to investigate the effect of bee pollen (BP) and black seeds (BS) as natural additive on blood biochemical and haematological, immunity response and diarrhea incidence of early weaning Friesian calves. Twenty-four newly born Friesian calves (12 males and 12 females) with average live body weight of 31 ± 0.26 kg were suckled their dam's colostrum for three days and divided into six similar groups (2 males and 2 females in each group) according to their live body weight and month of birth. The experimental period lasted 180 days over three consecutive periods (60 days each). All claves were fed a basal diet consisted of whole milk, calf starter and fresh berseem during suckling period and on calf starter and fresh berseem during 1st post weaning period or concentrate feed mixture, berseem hay and rice straw during 2nd post weaning period. Group1 given their diet without any additives and served as control, while G2 and G3 groups were supplemented with 5 or 10g BP/head/day in their diets, respectively. While, the diets of G4 and G5 groups were supplemented with 5 or 10 g BS/head/day, respectively. Lastly, G6 group was supplemented with 2.5 g BP + 2.5 g BS/head/day.

Results showed that G3 and G5 showed the highest concentrations of total protein and globulin in blood plasma followed by G2, G4 and G6, while G1 the lowest concentrations during suckling, 1st and 2nd post weaning periods. While, albumin concentration was nearly for the different groups. Plasma creatinine, ureaN concentrations as well as AST and ALT enzymes were significantly lower (P< 0.05) in G3 and G5 compared to G1, while in G2, G4 and G6 were intermediate with insignificant differences during the different periods. The counts of white blood cells, lymphocytes, monocytes and granulocytesin blood of Friesian calves improved significantly (P< 0.05) with BP and BS additives with the best results in G3 and G5 compared to G1 during experimental periods. Groups 3 and 5 recorded significantly (P< 0.05) the highest RBC's count, HGB concentration and HCT percentage followed by G2, G4 and G6, while G1 had the lowest values during the three periods. The high levels of BP and BS additives in G3 and G5 recorded significantly (P< 0.05) the highest values of MCV, MCH, MCHC and RDW, while the lowest values were in G1 during the different periods. Groups 3 and 5 (10g BP and BS) recorded significantly (P< 0.05) the highest values of PLT, PCT, MPV and PDW, while G1 had the lowest values during the different stages. Groups 3 and 5 recorded significantly (P< 0.05) the highest immunoglobulins (IgG, IgM and IgA) concentrations in plasma followed by G2, G4 and G6, while the lowest values were in G1 during the 3rd and 6th months of age. The BP and BS additive decreased significantly (P< 0.05) the percentage of diarrhea incidence during suckling and 1st post weaning periods with the lowest value in G5 and decreased gradually with advancing age from the birth to the fourth month of age. In conclusion, the high level of BP and BS additives (10 g/head/day) improved blood biochemical, haemotological and immunoglobulins as well as reduced diarrhea incidence of suckling and post weaning Friesian calves.