Genetic variability within the species has been efficiently utilized by breeders in their efforts to improve crops. However, the existing variability in a breeding population may not be sufficient for modern plant breeding purposes, and thus great efforts have been made to broaden the existing gene pool of crops. Introduction of new traits has been based mainly on sexual crosses between different genotypes within or between closely related species. However, due to the presence of various reproductive barriers, gene transfer has been restricted to sexually-compatible species, thus limiting the possibilities of modifying and improving crop plants. Many desirable and agronomically-interesting traits may only be found in distantly related species or even in unrelated organisms. Since they constitute a genetic resource potential, considerable effort has been allocated to identify and isolate these genes and transfer them into crops. Through the rapid development of somatic cell genetics, methods now exist for transferring genes across sexual borders and over wide taxonomic distances. Besides being of value for the transfer of unidentified genes, somatic hybridization is a tool for the modification and improvement of polygenic traits. Furthermore, the modification of organellar genetic material is possible via somatic hybridization since a mixture of the two fusion partners is obtained in the hybrid cell Production of hybrid plants through the fusion of protoplasts of two different plant species/varieties is called Somatic Hybridization, and such hybrids are called Somatic Hybrids. Therefore, somatic hybridization can be resorted to only when the following two criteria are satisfied: i) Isolation of protoplast in large quantity, and ii) Totipotency of the isolated protoplasts. In general, Somatic hybridization is an important tool of plant breeding and crop improvements through the production of inter-specific and inter-generic hybrids. It is valuable for plants which is asexual, sterile and that is also beneficial for those plant which has sexual incompatibility with other species.