The purpose of this research is to examine the effect of dependent variables of organizational culture, emotional intelligence, and job satisfaction with an independent variable, lecturers' performance when tested both individually or altogether. The survey is conducted by taking a sample of 84 lecturers of 4 (four) Theological Colleges by using proportional random sampling technique in Bogor and Bekasi West Java, Indonesia. The research used a Sequential Explanatory Design Mixed Method where quantitative research is conducted first then followed by the qualitative research. The study both quantitatively and qualitatively reveals that there is a positive significant relationship among variables under the following distribution of coefficient of correlation: organizational culture to lecturers' performance = 0.552, emotional intelligence to lecturers' performance = 0.587, job satisfaction to emotional intelligence = 0.609 and when tested together it produces coefficient of correlation = 0.688 and coefficient of determination = 0.473 indicating the existence of other 52.7% variables not including in the model affecting lecturers' performance.