The objective of this research is to determine the correlation between dependent variables of organization culture, transformational leadership, and job motivation with an independent variable; organizational commitment of lecturers in a state institute for Islamic study.. The survey is executed on samples of 180 lecturers by proportional random sampling technique in Jambi, Indonesia. The research applies a Sequential Explanatory Design Mixed Method by conducting quantitative research first then followed by the qualitative research to obtain comprehensive output. The study both quantitatively and qualitatively reveals that there is a positive significant relationship among variables under the following distribution of coefficient of determination: organizational culture to lecturers' organizational commitment = 17 %, transformational leadership to lecturers' organizational commitment = 18 % , working motivation to lecturers' organizational commitment = 14 % and when tested together it produces coefficient of correlation = 19 % indicating the existence of other 81 % variables not including in the model affecting factors affecting organizational commitment in a state institute for Islamic study's lecturers working environment.