A comparative assessment of the effects of some physico-chemical parameters on breeding of catfish (Clarias gariepinus) using ovaprim and ovatide was investigated from the month of July to August, 2016 in Makurdi, Benue State, Nigeria. Twelve (12) female brooders and six (6) males were obtained from Ocepson's Farm and Aqua-heaven Fish Farm respectively in Makurdi. The doses of 0.8ml/kg, 0.5ml/kg and 0.3ml/kg of ovaprim and 0.3ml/kg, 0.2ml/kg and 0.1ml/kg of ovatide were used. Some physico-chemical parameters such as dissolved oxygen, temperature and pH were monitored during breeding of the fish. The maximum mean temperature of 26.43°C was observed in 0.3ml/kg dosage of ovaprim while in ovatide, the maximum mean temperature of 26.90°C was observed in a dosage of 0.1ml/kg. There was no significant difference (p>0.05) in the mean temperature amongst all the varying doses of the hormones used. The maximum mean pH of 7.01 was observed in 0.8ml/kg dosage of ovaprim whereas a maximum mean pH of 6.99 was observed in a dosage of 0.3ml/kg of ovatide. There was no significant difference (p>0.05) in the mean water pH amongst the varying doses of the hormones used. The minimum and maximum mean incubation period of 20.66±40 and 23.00±25.00 hours respectively were observed in ovaprim. The minimum and maximum mean incubation period of 20.83±38.00 and 22.83 hours respectively were observed in ovatide. There was a significant difference (p< 0.05) in the mean incubation period of the eggs that were induced with varying doses of ovaprim and ovatide.