In this paper,5 years old "Kyoho" grape were taken as research object to study the effect of sheep manure and chemical fertilizer on growth and fruit quality of grape using different sheep manure, urea and diammonium phosphate orthogonal design. The results show that different sheep manure and chemical fertilizer combination had significant effects on grape branches length and diameter, internode length, fruit length, fruit diameter and other growth indexes and grape seed weight, soluble solids, soluble sugar content and titratable acid content and sugar acid fruit quality index were also significant different. A comprehensive analysis and evaluation of the indexes of grape growth and grape quality demonstrated that the optima sheep manure and chemical fertilizer combination for grape cultivation were in the order: the combination with Sheep (60t/ hm2) + urea (0.4t/ hm2) + diammonium phosphate (0.5t/ hm2), sheep (40t/ hm2) + urea (0.4t/ hm2) + diammonium phosphate (0.5t/ hm2) and sheep (60t/ hm2) + urea (0.4t/ hm2) + diammonium phosphate (0.4t/ hm2)