Orchis pinetorum, Anacamptis pyramidalis, and Dactylorhiza nieschalkiorum protocorms and plantlets were obtained via asymbiotic germination under in vitro conditions. For the germination of seeds, 4 basic nutrient media consisting of half-strength MS (1/2MS), full-strength MS, Van Waes DeBergh (VW and DB), and Knudson C. The petri dishes contained orchid seeds and the nutrient medium semi-solidified with agar (0.6%). cultures were incubated in darkness for the first 3 months and thereafter transferred to a light/dark photoperiod of 16/8 hours. At the end of the third month, protocorm rates were identified. They are subcultured every 4 weeks and 1/2 MS medium was used as a transfer medium. The highest germination rate,protocorm rate and plant growth rate were obtained from Knudson C medium.