In the fast developing era urbanization takes place very rapidly and it is directly or in directly harmful for environment. Most common construction material cement mortar contains sand which is natural resource and as demand of sand (fine aggregate) increases scarcity of natural sand is the main concern. On the other hand aggregate crushing plant has a byproduct "Quarry Dust". Replacement of quarry dust by natural sand in cement mortar is done after comparing physical and chemical properties of natural sand and quarry dust. The results shows that it has almost similar properties so in this research natural sand is 100% replaced by quarry dust and in addition inclusion of bacteria (Bacillus Pesteurii) in different concentration like; 105 ,106, 107,108 cell/ml is done. As Bacillus Pesteurii can produce calcium carbonate (CaCO₃) as a filler material and serve as a binding factor in cement mortar. This study shows that the compressive strength is increases of the bacterial mortar than the standard mortar or sustainable mortar. The results of compressive strength test are higher of the cell concentration 107 cell/ml that is 31.01 N/mm². So a sustainable cement mortar which is economical can be used to utilize waste and protection of environment can be done.