Hepatocellular carcinoma (HCC) is one of the most common human malignancy cancers and lacks the specific diagnosis targets and good treatment method. Herein, two abnormal expressed proteins in tumour cells were reported which can affect cell proliferation by proteomics technology combined with bioinformatics analysis methods. The QSG-7701 hepatocyte cell line and QGY-7703 hepatocyte cancer cell line which have the same genetic background were used as experiment samples, the mechanisms of tumour development and apoptosis induced by cisplatin (CDDP) were researched at the level of isolated culture cells. The QGY-7703 apoptosis model by CDDP were established and compared with QSG-7701 and QGY-7703 in morphology and growth characteristics. Then the proteins were extracted from these samples and the differentially expressed proteins were inspected thought 2-DE software analysis. Finally, two target proteins which were specially expressed in QGY-7703 and lacked in QSG-7701 and QGY-7703 after treatment with CDDP, were certified and analysed by mass spectrometry technology and bioinformatics method.