An attempt to establish a reference range for serum creatinine using Uncompensated Jaffe's Kinetic method having calibrators traceable to IDMS, referenced to SRM 967 in Government Kilpauk Medical College Hospital Laboratory , Chennai, South India was made involving 254 healthy adult individuals. They were assessed for liver and renal functions. The reference range for creatinine was 1.15 - 1.24 mg/dl for men and 0.96 - 1.04 mg/dl for women. The creatinine levels were not correlating with diet and age, but were correlated positively with mid arm circumference and gender. The derived reference range, deviated significantly from the global reference ranges, the range being very narrow and females having levels of creatinine higher than the globally reported range, could possibly be explained by the correlation of creatinine with ethnicity. The eGFR values calculated from the IDMS creatinine values, also showed narrow reference ranges in comparison to a similar study done in south Indian population at CARE Hospitals, Banjara Hills, Andhra Pradhesh. These newly established values of serum creatinine and eGFR may have their implications in the form of resetting standards for diagnosis of CKD in our population, if validated through multi-centric trials.