Submit Paper

Article Processing Fee

Pay Online

           

Crossref logo

  DOI Prefix   10.20431


 

International Journal of Advanced Research in Chemical Science
Volume 6, Issue 5, 2019, Page No: 13-18

Determination of Quinolones in Human Urine and Water Samples by Ultrasound Assisted Dispersive Liquid-Liquid Microextraction Based on Solidification of Floating Organic Droplet Followed High Performance Liquid Chromatography

Sen Lv1, Yiqing Sun1, Ying Yang1, Zongliang Niu1, Yingying Wen2*

1.Department of Environmental Science, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China.
2. Laboratory of Pathogenic Biology and Immunology, School of Basic Medical Science, Hainan Medical University, Haikou 571199, China.

Citation : Yingying Wen,et.al., Determination of Quinolones in Human Urine and Water Samples by Ultrasound Assisted Dispersive Liquid-Liquid Microextraction Based on Solidification of Floating Organic Droplet Followed High Performance Liquid ChromatographyInternational Journal of Advanced Research in Chemical Science 2019, 6(5) : 13-18.


Abstract

A ultrasound-assisted dispersive liquid-liquid microextraction based on solidification of floating organic droplet (UDLLME-SFO) combined with high performance liquid chromatography (HPLC) method was developed for the determination of quinolones (QNs) in human urine and water samples. The parameters of UDLLME-SFO procedure including sample pH, type and volume of disperser solvent, type and volume of extraction solvent and ultrasound time were optimized. The optimal extraction conditions of UDLLME-SFO were: sample pH 5; extraction solution, 300 µL 1-dodecanol; dispersion solution, 200 µL methanol; ultrasound time, 10 min. Under the optimum extraction and determination conditions, good responses for QNs were obtained in a range of 1-15 µg/mL, with linear coefficients greater than 0.97. The recoveries of the quinolones ranged from 66% to 102%. The limits of detection based on a signal-to-noise ratio of 3 was 0.03 µg/mL. The method was applied to the determination of QNs in human urine, tap and lake water samples with a satisfactory result. The results demonstrated that this UDLLME-SFO-HPLC method was successfully applied to determine QNs in urine and water samples.


Download Full paper: Click Here