The formation of ethanol from food such as corn, sugarcane etc. is a popular method of making ethanol. This study explains using renewable feed for the production of ethanol from cellulose. Rice husk and water hyacinth were used to carry out the experiment for the manufacture of ethanol. The powdered rice husk and water hyacinth were exposed to physical pretreatment and chemical pretreatment prior to enzymatic and hydrolysis process. Physical pretreatments and chemical pretreatment (acid pretreatment) were used to enhance the conditions for maximum ethanol yield from rice husk and water hyacinth. As a result of qualitative test, pretreatment methods had been useful on rice husk and water hyacinth in order to upsurge the available cellulose amount and also improve the enzymatic hydrolysis efficiency. The FTIR study revealed that characterization of pretreated rice husk and water hyacinth and suggests that both samples contain a substantial amount of ethanol. Pretreatment aided the highest sugar yield from enzymatic hydrolysis of pretreated rice husk and water hyacinth. Hence pretreated rice husk and water hyacinth could be useful as a carbon source for subsequent bio-ethanol production.