The Baltic amber extract was prepared and it was firstly used to react with BSA in order to develop the medicinal value of the amber. Fluorescence quenching spectroscopy found that the fluorescence intensity of BSA gradually decreased with the increase of the amber extract and the peak of the maximum excitation light of BSA was blue-shifted. Meanwhile, the quenching constants obtained decreased with increasing temperature, which indicated the quenching mechanism was static. The result from Rayleigh Scattering (RLS) Spectroscopy also showed the same quenching mechanism, Finally Synchronous fluorescence investigated that the binding site of amber extract with BSA was closer to tryptophan.