Abstract

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Under acidic conditions, monodisperse micron sized mesoporous silica which had an about 1.4 μm diameter, was synthesized by hydrothermal method using F127 (EO₁₀₆PO₇₀EO₁₀₆) as template and tetraetho- xysilane (TEOS) as silica source. The surface area and pore volume were determined by nitrogen adsorption method, and the diameter of the mesoporous silica was obtained by field-emission scanning electron microscopy. Then the mesoporous silica was used firstly as a carrier for the immobilization of Β-galactosidase from aspergillus oryzae, under the optimum conditions, the enzyme activity and the activity yield were 355.29 U/g and 63.11%, separately. Meanwhile, the fundamental properties of the immobilized enzyme, such as the influences of temperature and pH, were assayed and valuable results were obtained.

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