The Objective of the current study was quantification of orlistat by a validated, simple and sensitive high performance thin layer chromatographic-densitometric assay method. Separation of Orlistat was carried out on silica gel 60F254, 10 * 10 cm²thin layer chromatography (TLC) plates with 6 mm band length and 10 μl injection volume. Development chamber was saturated for 30 min. prior. Ascending chromatography TLC plate developed using mobile phase containing n-hexane, ethyl acetate, glacial acetic acid (7:3:0.1 v/v/v) respectively in glass twin-trough development chamber. The plates were developed up to a distance of 80 mm at temperature of 25°C and dried with hair drier at 50°C. Quantification was carried out with CAMAG TLC Scanner 3 operated by WinCats software using a deuterium lamp. The linear regression equation for Orlistat was y = 847.15x+3557.3 with correlation co-efficient 0.9984. The LOD value for Orlistat was found to be 1.14 μg/ml, and the LOQ value 3.81 μg/ml. The mean recovery for Orlistat was 99.13-99.92%. The %RSD values for intermediate and method precision study was 0.017% and mean % assay was 99.79% as well as absolute difference between mean % assay values of method precision and intermediate precision found 0.04 % for Orlitat. The effects of such deliberate changes on peak area and % assay were calculated, which found 99.60 % to 100.12 %. as well as % RSD for retardation factor found 0.67%. There was no indication of compound instability in the sample solution was found through the study.