Abstract

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The water vapor distillation method was used for extracting the volatile oil in Wampee fruits, the volatile oil was identified by thin layer chromatography (TLC), and the component extraction for volatile oil was determined by GCMS computer combined with identification instrument the composition and the peak area normalization method to get the relative content of each component. Results showed that the 25 peaks isolated were identified 23 components which are phellandrene (36.97%), terpinen-4-ol (17.58%), sabinene hydrate (13.76%). These three substances are relatively high. In the study of the antioxidant activity of the volatile oil, the effect of the scavenging rate of hydroxyl radical was studied. It was found that the concentration of volatile oil in a certain concentration range was positively correlated with the clearance rate of hydroxyl radical. The filter paper method for study the antimicrobial activity of the essential oil presented that it all had a certain antibacterial effect to Staphylococcus aureus, Bacillus subtilis and Escherichia coli. And inhibition of different concentrations of essential oils was different for different types of bacteria. This bacteriostasis becomes smaller as with the decrease of concentration. And the effect of essential oil on mouse pancreatic cancer cells was also studied. The experiments showed that the essential oil had an effect of tumor inhibition.

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