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DOI Prefix 10.20431 |
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International Journal of Advanced Research in Chemical Science
Volume-3 Issue-12, 2016, Page No:25-33
Application of 2-Chloro-7-Nitrobenzo-2-Oxa-1, 3-Diazol (NBDCL) for Spectrophotometric Determination of Finasteride in Pharmaceutical Formulation
Sally Mohammed Ahmed1, Abdalla Ahmed Elbashir1*, Younis Mohamed Elhassan2
1.University of Khartoum, Faculty of Science, Chemistry Department, Khartoum, Sudan
2.University of Medical Sciences and Technology, Khartoum, Sudan
2.University of Medical Sciences and Technology, Khartoum, Sudan
Citation : Sally Mohammed Ahmed, Abdalla Ahmed Elbashir,Younis Mohamed Elhassan, Application of 2-Chloro-7-Nitrobenzo-2-Oxa-1, 3-Diazol (NBDCL) for Spectrophotometric Determination of Finasteride in Pharmaceutical Formulation International Journal of Advanced Research in Chemical Science. 2016;3(12):25-33.
Abstract
A simple, sensitive and validated spectrophotometric method for the determination of finasteride using 2-Chloro-7-nitrobenzo-2-oxa-1, 3-diazol (NBD-CL) has been developed. The method is based on the reaction of finasteride and NBD-CL at alkaline medium (pH 11.0) to develop a deep brown adduct that bears maximum absorption at 467 nm. Beer’s law is obeyed in the concentration range 25-75 μg/mL of finasteride at the selected wavelength. Under optimized reaction conditions, the linear regression equation of the calibration curve is y = 0.010489524x-0.012190476 with a linear regression correlation coefficient of 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.17 μg/mL and 0.52 μg/mL respectively. The method has been successfully applied to the determination of finasteride in pharmaceutical formulation.
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