Persian parrotia (Parrotia persica C.A. Mey.) is native to northern Iran and Azerbaijan, along the Caspian Sea. Persian parrotia is an important tree species in Hyrcanian forests and also well known for its landscape values and the wood is also used by wood turners and for weaving shuttles, telephone poles. Very limited agronomic information exists regarding the cultivation of Iron tree as a industrial crop. In this study we investigated effects of cold stratification (25, 50 and 75 days) in combination with putrescine (5, 10 and 15 mM), BA (1, 2 and 3 mM), KNO₃ (25, 50 and 75 mM) and warm stratification (42 days) on seed germination and emergence of P. persica and assessment of genetic diversity in it seedlings using RAPD markers. The results of germination treatments showed that the highest emergence percentage was obtained with 42 days warm moist chilling followed by 50 days cold stratification. To assessment of genetic diversity, 18 RAPD primers were used. The results indicated that 6 out of 18 primers produced no band on the studied genotypes and the rest primers (12) produced 92 bands overall. The average polymorphism bands were 7.66%. The lowest number of bands was three bands with 66.6% polymorphism which obtained with OPB-10 primer and the highest number of polymorph bands was 12 bands with 100% polymorphism gained with OPD-05 primer. The highest resolving power (0.48 and 0.47) was found with OPA-10 and OPB-10 primers, respectively. The lowest resolving power (0.23) was related to OPE-06 and TIBMBC-13 primers. The molecular results showed that OPD-05 and OPA-10 and OPA09 primers can use for ongoing genetic variation studies in Persian parrotia.