An effective protocol for adventitious shoot proliferation was established on MS medium supplemented with different concentrations of cytokinin and auxin using leaf explants from in vitro grown shoots of Gynura porcambens. Highest frequency 86.67% of adventitious shoot formation, maximum number 20.52 of shoots per explant and average 7.3 cm length of shoot were obtained on MS medium supplemented with 4.0 μM 6-Benzylaminopurine (BAP) and 1-Naphthaleneacetic acid (NAA). Shoot multiplication and growth were significantly affected by different types of basal medium, level of concentration of sucrose, and pH. The highest rate (92.31%) of shoot production was achieved in a shoot-regenerating full strength of MS basal medium with 30 gm/l sucrose where pH was adjusted to 6.0. For rooting, the in vitro proliferated and elongated shoots were excised into 2-4 cm long microcuttings, which were planted individually on a rootinduction MS medium containing 4.0 μM Indole-3-butyric acid (IBA). Within 4 weeks of transfer to the rooting medium, all the cultured microcuttings produced 2-6 roots. The in vitro regenerated plantlets were transferred to vermicompost, and the survival rate underex vitro was 100.00%.