An efficient and reproducible in vitro regeneration protocol for a popular chili variety Bogurar jhal morich was established. For regeneration of shoots two different explants such as cotyledonary leaf and cotyledonary node were employed using various concentrations and combinations of different growth regulators. MS medium supplemented with 26.0 μM BAP was found best for shoot induction from cotyledonary node explants whereas in case of cotyledonary leaf explants, 26.0 μM BAP and 2.32 μM Kn hormonal combination produced best results. Cotyledonary node explants also responded best towards multiple shoot induction on MS medium supplemented with 2.72 μM TDZ. MS medium supplemented with 7.2 μM GA3 and 24.0 μM AgNO₃ were found to be the best media combinations for shoot elongation. Highest percentage of root induction was achieved on MS medium supplemented with 3.0 μM IAA from excised shoots of chili. Following the development of roots in vitro regenerated plantlets were successfully transplanted into soil.