New charophytes locality the Ein El Balad in the territory of the Mount Carmel Biosphere Reserve in Northern Israel was studied firstly with implementation bio-indication methods for revealing of algal diversity and ecological assessment of the water object environment. Altogether forty seven species of cyanobacteria and algae including one of them macro-algae Chara vulgaris Linnaeus were revealed. Chara was found in massive growth in two ancient pools. Bio-indication and chemical variables characterize the pools environment as eutrophic, moderate organic polluted, Class III-V of water quality. Water was fresh, temperate, low alkaline, and well saturated by oxygen. Succession of algal community shows increasing of organic pollution from dry to wet season as a result of groundwater impact which has nitrates concentration increasing after rainy period. Bio-indication demonstrated high self-purification capacity of the pools ecosystem. We can recommend the Ein El Balad pools for monitoring of natural aquatic object in the Mount Carmel Biosphere Reserve as reference site, and Chara vulgaris as climatic and successional indicator.