Echinacea commonly called the Purple coneflowers, is a genus of nine species of herbaceous plants in the Family Asteraceae. Three of them are important in commerce, with the majority of wild harvest being E. angustifolia. It has been used for a variety of ailments, including toothache, coughs, colds, sore throats, snakebite, and as a painkiller. In the current study, in vitro inhibitory activity of Echinacea angustifolia essential oils were screened against Coliform spp, Pseudomonas spp, Saccharomyces cerevisiae (EC1118), Zygosaccharomyces bailii (DSM 70492) and Lactobacillus plantarum (DSM2601). Agar well diffusion assay was adopted for the study. E. angustifolia oils showed very weak antimicrobial activity against the microorganisms tested with diameter of inhibition zone not exceeding 3 mm. The highest activities were observed for Z. baillii and S. cereviceae at a concentration of 10 and 100 ppm respectively, while for the rest of the strains the diameter of inhibition zone were ranged 1 and 2.5 mm, except Coliform spp which was not affected by the presence of the essential oil at a concentration of 50 ppm. The low bacteriostatic effect of this plant essential oil against some of the most important causes of infections provides an exciting potential for the future, especially in the light of the shift away from commonly used antibiotics and the move towards more natural alternatives.