Based on the new research of possible entropy decrease in isolated system under existence of internal interactions, the biological systems possess many internal interactions, in which entropy decrease is possible. Further, we propose the universal formula on entropy change in any systems for outside and inside. Systems biology and evolution are discussed. Life always changes from every individual to total system for various levels and different times. This not only input energy, and more is determined by tissues, structures and interactions inside system. Inside and outside combination determines the growth and evolution of life system. From this health is researched. It is not possible that self-assembly, self-organization, self-production, self-fabrication, self-maintaining, etc., all are always entropy increase. The biological membrane is already a natural existent Maxwell demon. Thermodynamics as science must be developed, from this will accelerate the next great advance.