Personality traits in marriage relationship conceptualized as autonomy, aggression, conformity, conflict-orientation, & compromise are culturally constructed that may vary across the cultures, or subcultures within a given society. This study further explores and compares personality traits in marital relationship for practical implications between Muslim and Santal communities in Bangladesh. For this research purpose we selected 200 couples (120 couples for Muslim and 80 couples for Santal) using purposive sampling from Rajshahi city, Bangladesh and interviewed through structural questionnaire. The results of Independent Samples t-test revealed that there were significant differences in personality traits in marital relationship: autonomy, aggression, conflict-orientation, and compromise except conformity between the communities' couples in the study area of Bangladesh. The findings may have implications in further research and family social work practice with the communities' couples in Bangladesh.