Metals occur naturally in the earth's crust and their contents in the environment can vary between different regions resulting in spatial variations of background concentrations. Human health is closely linked with a healthy and clean environment. Rapid growth in urbanization and industrialization has increased the levels of heavy metals in the environment and consequently in the food chain. Metal ions are required for many critical functions, physiological and pathological process in human. Iron and Manganese are essential micronutrients with a variety of biochemical functions in all living organism and their lethal dose become toxic to human body. High concentration of metal ions in living system can be prevented by the use of specific chelating agents. The complexation of Fe(III) and Mn(II) with medicinally important ligand ephedrine and NTA in solution were studied by paper electrophoretic technique. Stability constants of investigated chelates were determined at 25°C. The stability constant data revealed that ephedrine and NTA may be used as chelating agent.