The chemical analysis of bio-sap of medicinal plants Hibiscus Rosa Sinenses-L (HRS) of malvaceae family have been performed chromatographically and documented. The work is restricted to the identification of pigments, and free amino acids. Various physical parameters related to analysis were determined. Electrophoretic method was employed to measure the relative motion of ions of amino acids. The fluorescent and UV spectroscopic methods were used for the active ingredient's analysis.